Quantification of Proliferation and Viability within Spheroids
3:33
Analysis of Cancer Stem Cell Populations with Flow Cytometry
5:15
FACS Data Analysis
8:03
Results: Patient-derived Cancer Stem Cells within 3D Hanging Drops
9:03
Conclusion
副本
Our protocol provides a simple method for 3D cell culture and is highly amenable to downstream analysis. This facilitates the evaluation of heterogeneous patient samples and their specific drug responses. The advantage of this technique is that sp
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This protocol describes generation of patient-derived spheroids, and downstream analysis including quantification of proliferation, cytotoxicity testing, flow cytometry, immunofluorescence staining and confocal imaging, in order to assess drug candidates’ potential as anti-neoplastic therapeutics. This protocol supports precision medicine with identification of specific drugs for each patient and stage of disease.
