Human Umbilical Cord Blood Mononuclear Cell (MNC) Isolation
2:45
CD34+ Cell Isolation from MNCs
4:55
Flow Cytometric Analysis of Hematopoietic Stem and Progenitor Cell (HSPC) Populations
5:59
CD34+ HSPC Myeloid Differentiation
8:06
Results: Representative Characterization of Freshly-isolated and Differentiated HSPCs
9:47
Conclusion
副本
This protocol can be used to study the molecular and cellular changes that occur during the normal myeloid differentiation of human CD34 positive hematopoietic stem and progenitor cells. The main advantage of this protocol is that it allows the di
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Here, we present a protocol for immunophenotypic characterization and cytokine induced differentiation of cord blood derived CD34+ hematopoietic stem and progenitor cells to the four myeloid lineages. The applications of this protocol include investigations on the effect of myeloid disease mutations or small molecules on myeloid differentiation of the CD34+ cells.
