Results: Representative Nuclear Magnetic Resonance (NMR)-Based Assay Results
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Conclusion
副本
NMR-based activity assays provide an effective method for identifying, evaluating, and validating inhibitors for a given enzyme. They are particularly well-suited for fragment screening. NMR assays are amenable to the higher compound concentration
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NMR-based activity assays have been developed to identify and characterize inhibitors of two nucleoside ribohydrolase enzymes. Protocols are provided for initial compound assays at 500 μM and 250 μM, dose-response assays for determining IC50 values, detergent counter screen assays, jump-dilution counter screen assays, and assays in E. coli whole cells.