Isolation of GFP-positive Cells via Flow Cytometry
4:20
Extraction of RNA from Isolated Cells
6:55
Results: Isolation of Specific Neuron Populations
8:40
Conclusion
副本
This protocol allows for the seamless isolation, culturing, and interrogation of transcriptional responses in a specific cell lineage from C.elegans. The main advantage of this protocol is the high level of adaptability to different life stages of
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Here, we present a protocol for simple isolation of specific groups of live neuronal cells expressing green fluorescent protein from transgenic Caenorhabditis elegans lines. This method enables a variety of ex vivo studies focused on specific neurons and has the capacity to isolate cells for further short-term culturing.
