Results: Cell Type-specific Isolation of High-quality RNA by Trap
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Conclusion
副本
With TRAP-seq, we can isolate the RNA from specific cell types, such as the hepatocytes repopulating in injured liver to analyze the gene expression changes occurring in those cells. It does not require any steps to remove unwanted cells from the
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Translating ribosome affinity purification (TRAP) enables rapid and efficient isolation of cell type-specific translating mRNA. Here, we demonstrate a method that combines hydrodynamic tail-vein injection in a mouse model of liver repopulation and TRAP to examine the expression profile of repopulating hepatocytes.
