Green Dendra2 Conversion: Data Acquisition at Time Zero
5:35
Red Dendra2 Conversion: Data Acquisition at Second Time Point
6:13
Converted Dendra2 Image Analysis
7:17
Results: Representative Huntingtin (HTT)-D2 Conversion and Degradation
9:08
Conclusion
副本
This protocol allows for tracking and quantification of the degradation of a protein of interest in live and aging C.elegans. This in vivo and noninvasive technique requires only little microscopy set up to reliably convert Dendra2 into a bright a
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Presented here is a protocol to monitor degradation of the protein huntingtin fused to the photoconvertible fluorophore Dendra2.
