Equilibrating Materials to Experimental Temperature
2:06
Inoculation of Agarose Pads
3:13
Microscope Setup for Live Imaging
5:32
Results: P. aeruginosa Behavior when Cocultured with S. aureus
7:47
Conclusion
副本
This protocol allows for visualization and tracking of interspecies bacterial interactions at the single cell level over time. This techniques main advantage is its applicability to studying bacterial interactions, including cell tracking, gene ex
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This live-bacterial cell imaging protocol allows for visualization of interactions between multiple bacterial species at the single-cell level over time. Time-lapse imaging allows for observation of each bacterial species in monoculture or coculture to interrogate interspecies interactions in multispecies bacterial communities, including individual cell motility and viability.
