Immobilized Metal Ion Affinity Chromatography (IMAC) and High pH Reversed-Phase (Hp-RP) Stage Tip Preparation
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IMAC Stage Tip Phosphopeptide Enrichment
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Hp-RP C18 Stage Tip Phosphopeptide Fractionation
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Results: Representative Osmotic Stress Signaling in Arabidopsis
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Conclusion
副本
This strategy is a powerful tool for comprehensively studying the global changes of plant phosphoproteomes, in response to biotic or abiotic stresses. This technique allows in-depth phosphoproteomics analysis, in an easy-to-use and high throughput
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Presented here is a phosphoproteomic approach, namely stop and go extraction tip based phosphoproteomic, which provides high-throughput and deep coverage of Arabidopsis phosphoproteome. This approach delineates the overview of osmotic stress signaling in Arabidopsis.