Results: Representative LCM Sample Acquisition and RNA Quantification
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Conclusion
副本
This protocol uses Laser-Capture Microdissection coupled with RNA-Sequencing to obtain Spatial and Temporal transcriptomes from specific cells in plants of interests, using small quantities of biological materials. The main advantage of laser tech
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Presented here is a protocol for laser-capture microdissection (LCM) of plant tissues. LCM is a microscopic technique for isolating areas of tissue in a contamination-free manner. The procedure includes tissue fixation, paraffin embedding, sectioning, LCM and RNA extraction. RNA is used in the downstream tissue-specific, temporally resolved analysis of transcriptomes.