Results: Representative Virus Load and Survival Curve Analyses
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Conclusion
副本
Our protocol demonstrates how to produce honey bee virus particles en masse for their use in controlled, high-throughput bioassays for rapid screening of viral treatment effectiveness. The assay uses easy-to-culture bee pupae rather than cell cult
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Here we describe two protocols: first to propagate, extract, purify, and quantify large quantities of honey bee non-enveloped virus particles, including a method for removing honey bee pupae and second to test the effects of viral infection using a highly repeatable, high-throughput cage bioassay.