RNA Extraction of Collected Fractions (CE, FT, W and E)
5:24
Results: Constructs Validation and MAPS Controls
7:49
Conclusion
副本
MS2-affinity purification coupled with RNA sequencing or MAPS has been developed to identify the whole targetome of any RNA of interest in bacteria. This technology makes it possible to identify any kind of sRNA partners independently of the mecha
Sign in or start your free trial to access this content
MAPS technology has been developed to scrutinize the targetome of a specific regulatory RNA in vivo. The sRNA of interest is tagged with a MS2 aptamer enabling the co-purification of its RNA partners and their identification by RNA sequencing. This modified protocol is particularly suited for Gram-positive bacteria.
