Schneiders Line 2 Plus (S2R+) Drosophila Cell Fixation
2:02
Lysis
3:18
Enzymatic Digestion, DNA End Biotinylation, and Ligation
4:37
Crosslink Reversal and DNA Purification
5:33
Hi-C Template Quality Evaluation
6:26
Biotin Removal/End Repair and Size Selection
7:49
Biotin Pulldown/A-Tailing/Adapter Ligation
9:33
Results: Representative In-Nucleus Hi-C Analysis
11:17
Conclusion
副本
The nucleus Hi-C protocols allow the exploration of chromosomal confirmation at different resolution for the characterization of chromatin loops, domains, and compartment organizing the genome. This protocol provides high-resolution profiling of g
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The genome is organized in the nuclear space into different structures that can be revealed through chromosome conformation capture technologies. The in-nucleus Hi-C method provides a genome-wide collection of chromatin interactions in Drosophila cell lines, which generates contact maps that can be explored at megabase resolution at restriction fragment level.
