Designing of sgRNAs and Gene Disruption in Primary Human T-Cells
3:23
CRISPR Efficiency: Sequencing and Indel Detection
4:13
Results: Manufacturing Human CAR-T Cells Using CRISPR Cas9 Edited T-Cells
5:30
Conclusion
副本
Our laboratory protocol enables the combination of human genome editing with CAR T cell therapy. The laboratory procedure is the approach with CRISPR-Cas9 at targeting up to three genes with high efficiency. And finally, our approach is able to be
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Here, we present a protocol for gene editing in primary human T cells using CRISPR Cas Technology to modify CAR-T cells.
