This protocol resolves common challenges experienced with sputum processing that have made it difficult in the past to use with flow cytometry for high-throughput clinical diagnostic purposes. This protocol is designed to save time by using sputum
登录或开始您的免费试用以访问此内容
This protocol describes an efficient method for dissociating sputum into a single cell suspension and the subsequent characterization of cellular subsets on standard flow cytometric platforms.