Extracellular Vesicle (EV) Isolation and On-Chip Immuno-Fluorescent Labeling
2:54
Incubation of the Cells with Fluorescently Labeled EVs for the EV-Uptake Assay
3:45
Confocal Microscopy and Image Processing
6:28
Results: EV Uptake Assay Using 3D Fluorescence Imaging via Confocal Microscopy
7:31
Conclusion
副本
This protocol provides a robust methodology for analyzing EVs at a cellular level, and a practical approach for efficient EV uptake and EV tracking analysis. This method of EV labeling eliminates co-precipitation of EVs and dye, thus enhancing the
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Extracellular vesicles (EVs) contribute to cellular biology and intercellular communications. There is a need for practical assays to visualize and quantify EVs uptake by the cells. The current protocol proposes the EV uptake assay by utilizing three-dimensional fluorescence imaging via confocal microscopy, following EV isolation by a nano-filtration-based microfluidic device.