Collection of Young Adult Strongyloides for Microinjection
3:19
Preparing the Microscope and Breaking the Needle
5:27
Microinjecting Strongyloides
7:22
Collecting and Screening F1 Larvae to Recover Transgenics/Knockouts
8:31
Results: Generating Transgenics and Knockouts in Strongyloides Species Using Intragonadal Microinjection
9:04
Conclusion
副本
Using microinjection to deliver DNA constructs into Strongyloides allows us to generate knockouts and transgenics, which can be used to study how these worms develop, navigate their environment, and parasitize hosts. So far, this is the only succe
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The functional genomic toolkit for the parasitic nematodes Strongyloides stercoralis and Strongyloides ratti includes transgenesis, CRISPR/Cas9-mediated mutagenesis, and RNAi. This protocol will demonstrate how to use intragonadal microinjection to introduce transgenes and CRISPR components into S. stercoralis and S. ratti.
