Mass Spectrometry (MS) Sample Preparation with a Single Protein
4:44
Peptide Clean-up Using SP3 Paramagnetic Beads
6:19
MS Sample Preparation with Protein Lysate
8:37
Results: Mass Spectrometry Proteomics with a Single Protein Standard Bovine Serum Albumin and a Detergent-Containing Human Heart Lysate Sample
9:44
Conclusion
副本
This protocol uses automation to perform protein biochemistry preparations prior to mass spectrometry experiments, allowing higher throughput and lower variability for proteomic studies. This protocol uses a low-cost, programmable liquid handling
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Detailed protocol and three Python scripts are provided for operating an open-source robotic liquid handling system to perform semi-automated protein sample preparation for mass spectrometry experiments, covering detergent removal, protein digestion, and peptide desalting steps.
