Calibration and Preparation of the NMR Spectrometer for Acquisition
3:22
Animal Preparation, Surgical Procedure, and Perfusion of the Heart in the NMR Tube
9:23
Acquiring Data for Cardiac Energetics and pH
10:09
DNP Spin Polarization and Dissolution
11:25
Hyperpolarized 13C Spectroscopy
12:07
Representative Results
14:40
Conclusion
副本
Hi.In this video you will learn how to perform hyperpolarized MR studies of the perfused mouse heart. David Shaul, an MD/PhD student in the lab, will guide you through the various techniques and procedures. Today we learn a protocol for studying h
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We describe an experimental setup for administrating hyperpolarized 13C-labeled metabolites in continuous perfusion mode to an isolated perfused mouse heart. A dedicated 13C-NMR acquisition approach enabled the quantification of metabolic enzyme activity in real-time, and a multiparametric 31P-NMR analysis enabled the determination of the tissue ATP content and pH.