Discovering Novel Putative Neuropeptides Using De Novo Sequencing and Transcriptome Mining for Predicted Neuropeptide Sequences
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Results: De Novo Sequencing and MALDI-MS Imaging of Neuropeptides
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Conclusion
Transcript
The analysis of endogenous peptides, which are smaller than most proteins, but larger than many digested peptides is an under examined field. This protocol addresses the scap in mass spectrometry based workflows. Mass spectrometry is highly sensit
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Mass spectrometric characterization of neuropeptides provides sequence, quantitation, and localization information. This optimized workflow is not only useful for neuropeptide studies, but also other endogenous peptides. The protocols provided here describe sample preparation, MS acquisition, MS analysis, and database generation of neuropeptides using LC-ESI-MS, MALDI-MS spotting, and MALDI-MS imaging.
