Administration of Fe‐Transferrin Intravenously to E17.5 Pregnant Mice
5:53
Tissue Processing for Quantitative Iron Analysis by Inductively Coupled Plasma Mass Spectrometry (ICP‐MS)
7:14
Results: Physiological Regulation of Mouse Placental Ferroportin During Maternal Iron Deficiency
8:08
Conclusion
副本
This protocol is significant because it enables researchers to track and quantitate iron distribution in vivo without the need for radioactivity. Since multiple stable iron isotopes can be detected concurrently within the same sample, this techniq
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This article demonstrates how to prepare and administer transferrin-bound nonradioactive isotopic iron for studies of iron transport in mouse pregnancy. The approach for quantifying isotopic iron in fetoplacental compartments is also described.
