Dissection and Isolation of Mouse Cerebral Arterioles
2:30
Digestion of Parenchymal Arterioles and Preparation of Endothelial Tubes
3:47
Utilization of Arteriolar Endothelial Tubes for the Examination of Cellular Physiology and Cellular Imaging
4:54
Results: Assessment of Endothelial Function
5:50
Conclusion
副本
This method will provide better insight into the function of parenchymal arteriolar endothelium which directly links intracerebral blood flow to the fueling of neuronal and glial activity throughout the brain. A distinct technical advantage over a
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Intensive preparation of intact mouse cerebral endothelial "tubes" from cerebral parenchymal arterioles is illustrated for studying cerebral blood flow regulation. Further, we demonstrate the experimental strengths of this endothelial study model for fluorescence imaging and electrophysiology measurement of key cellular signaling pathways, including changes in intracellular [Ca2+] and membrane potential.
