Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform (RpEGEN) for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium

1:00

Screening Zebrafish Larvae for rpe65a:nfsB-eGFP and Genetic Ablation of the Retinal Pigment Epithelium

3:02

Incorporating Pharmacological Treatment into Zebrafish Retinal Pigment Epithelium Ablation Protocol

6:35

Confocal Microscope Z-stack Image Preprocessing in FIJI (ImageJ)

8:19

Quantification and Visualization of RPE Regeneration Using RpEGEN Scripts

10:42

Results: RPE Expression, Ablation, Regeneration Quantification

12:39

Conclusion