Calibration of Polarographic Oxygen Sensors for High-Resolution Respirometry (HRR)
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Dissection and Permeabilization of the Sciatic Nerve
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Oxygen Consumption and Reactive Oxygen Species (ROS) Production Determination
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Results: Assessing the Mitochondrial Oxygen Consumption by the Permeabilized Sciatic Nerve
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Conclusion
副本
This protocol allows the evaluation of mitochondrial function inside of nerve by parcellation of the isolated mitochondria, a challenging procedure in a small tissue that contains a limited number of mitochondria. The technique provides a possibil
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High-resolution respirometry coupled to fluorescence sensors determines mitochondrial oxygen consumption and reactive oxygen species (ROS) generation. The present protocol describes a technique to assess mitochondrial respiratory rates and ROS production in the permeabilized sciatic nerve.
