Purification of the RNA Polymerase and Preparation of RNA Polymerase Stock
3:35
In Vitro Transcription with the Incorporation of Radiolabeled Nucleotides
5:35
Results: Analysis of RpoD Purification and In Vitro Transcription
6:43
Conclusion
副本
The in vitro transcription assay system for Borreliella burgdorferi provides a biochemical tool for investigators to study gene regulatory mechanisms and factors that govern the enzymatic activity of RNA polymerase. The system allows us to test ho
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In vitro transcription assays can decipher the mechanisms of transcriptional regulation in Borreliella burgdorferi. This protocol describes the steps to purify B. burgdorferi RNA polymerase and perform in vitro transcription reactions. Experimental approaches using in vitro transcription assays require reliable purification and storage of active RNA polymerase.
