Results: Comparison of TSS Enrichment Scores and Differential Peaks Obtained Using Optimized Cell‐Lysis Buffers, Cell Number Input, and Tn5 Concentration of ATAC‐Seq Libraries
6:33
Conclusion
副本
ATAC-seq allows us to identify the status of the open chromatin regions in the genome, which is often altered in the disease states compared to the normal condition. Fewer cell input, a simplified protocol of Tn5 digestion, followed by isolation o
Sign in or start your free trial to access this content
ATAC-seq is a DNA sequencing method that uses the hyperactive mutant transposase, Tn5, to map changes in chromatin accessibility mediated by transcription factors. ATAC-seq enables the discovery of the molecular mechanisms underlying phenotypic alterations in cancer cells. This protocol outlines optimization procedures for ATAC-seq in epithelial cell types, including cancer cells.
