Making Supported Bilayers on Glass Substrate by Vesicle Fusion
4:22
Microscope Setup and Single-Particle Imaging Measurements
7:26
Image Acquisition for Counting Subunits in a Protein Assembly
8:40
Results: Analyzing the Binding of Cytolysin A (ClyA) Protein and Mobility of DNA Tracer
10:10
Conclusion
副本
Cellular membranes are highly crowded due to the presence of embedded proteins and sugars. We demonstrate single molecule imaging on synthetic crowded supported lipid bilayers. This platform is useful for investigating the effect of crowding on me
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Here, a protocol to perform and analyze the binding, mobility, and assembly of single molecules on artificial crowded lipid membranes using single-molecule total internal reflection fluorescence (smTIRF) microscopy is presented.
