Fabrication of the Two-Piece Channel and Modular Platform Base
4:28
Injecting the COL1 Solution into the Microchannel and Removing the Cover for Cell Culture Applications
6:16
Results: Confocal Reflectance Images of COL1 Fibers
6:45
Conclusion
副本
This method allows us to engineer 3D collagen hydrogels with controlled levels of fiber alignment to model environments found in healthy and diseased tissue in the body. This technique provides a simple microfluidic approach to engineer aligned co
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This protocol demonstrates the use of a microfluidic channel with changing geometry along the fluid flow direction to generate extensional strain (stretching) to align fibers in a 3D collagen hydrogel (<250 µm in thickness). The resulting alignment extends across several millimeters and is influenced by the extensional strain rate.
