Preparation of the 96-Well Plate for the Metabolic Extracellular Flux Assay
1:52
Preparation and Seeding of Cells
3:52
Preparing Compounds in the Mitochondrial Stress Test Kit
4:25
Running the Mitochondrial Stress Assay
7:29
Results: Metabolic Flux Assay to Analyze Oxygen Consumption Rates (OCR) in Neutrophils
9:02
Conclusion
副本
This protocol focuses on quantifying mitochondrial respiration of mouse and human neutrophils, as well as neutrophil-like HL60 cells using the metabolic extracellular flux analyzer. This method allows us to quantify mitochondrial function in neutr
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We describe stepwise protocols measuring the mitochondrial respiration of mouse and human neutrophils and HL60 cells using the metabolic extracellular flux analyzer.
