Preparation for ROS and Mitochondrial Membrane Potential Determination
3:22
TUNEL Staining Assay for Apoptosis Rate Determination
4:10
Transfection of mCherry GFP-LC3B Adenovirus to Monitor Autophagic Flow
4:53
Immunofluorescence Staining for Detection of Parkin's Mitochondrial Translocation
5:42
Results: Effects of Crocetin on Oxidative Stress-Induced Damage in H9c2 Cardiomyocytes
7:10
Conclusion
副本
This protocol provides a reference for the technique approach to the study of autophage and it describes the entire experimental procedures in detail. The main advantage of this technique is that it allows the length observation of the change in a
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Based on in vitro experiments, this study revealed the mechanism of crocetin in repairing oxidative stress damage of cardiomyocytes by influencing mitophagy, in which the PINK1/Parkin signaling pathway plays an important role.
