Anmelden

National Cerebral and Cardiovascular Center Research Institute

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Yuji Nashimoto¹, Yukako Teraoka¹, Ramin Banan Sadeghian¹, Akiko Nakamasu², Yuichiro Arima³, Sanshiro Hanada³, Hidetoshi Kotera¹, Koichi Nishiyama³, Takashi Miura², Ryuji Yokokawa¹

¹Department of Micro Engineering, Kyoto University, ²Graduate School of Medical Sciences, Kyushu University, ³International Research Center for Medical Sciences (IRCMS), Kumamoto University

The protocol describes how to engineer a perfusable vascular network in a spheroid. The spheroid's surrounding microenvironment is devised to induce angiogenesis and connect the spheroid to the microchannels in a microfluidic device. The method allows the perfusion of the spheroid, which is a long-awaited technique in three-dimensional cultures.

Medicine

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

Daiki Seya¹, Yuqing Xu^2,3, Toshifumi Mukunoki⁴, Kenichi Tsujita³, Osamu Nakagawa¹, Yuichiro Arima^1,2,3,4

¹Department of Molecular Physiology, National Cerebral and Cardiovascular Center Research Institute, ²International Research Center for Medical Sciences (IRCMS), Kumamoto University, ³Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, ⁴X-Earth Center, Faculty of Advanced Science and Technology, Kumamoto University

Here, we describe a visualization and quantification method for murine hind-limb vessels using micro-X-ray computed tomography.

Developmental Biology

Manipulating Mechanical Forces in the Developing Zebrafish Heart Using Magnetic Beads

Christina Vagena-Pantoula^1,2, Hajime Fukui^3,4, Julien Vermot^1,2

¹Department of Bioengineering, Imperial College London, ²The Francis Crick Institute, ³Institute of Advanced Medical Sciences, Tokushima University, ⁴Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute

This protocol outlines a method for grafting a magnetic bead into the developing zebrafish heart through microsurgery, enabling the manipulation of mechanical forces in vivo and triggering mechanical stimulus-dependent calcium influx in endocardial cells.