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Azabu University

3 ARTICLES PUBLISHED IN JoVE

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Behavior

Determining Ultrasonic Vocalization Preferences in Mice using a Two-choice Playback Test
Akari Asaba 1, Masahiro Kato 3, Nobuyoshi Koshida 2, Takefumi Kikusui 1
1School of Veterinary Medicine, Azabu University, 2Division of Electric and Information Engineering, Tokyo University of Agriculture and Technology, 3Kato Acoustics Consulting Office

Ultrasonic vocalizations (USVs) in mice differ depending on age, sex, condition, and genetic background. Using two ultrasound emitters broadcasting simultaneously in different locations, this two-choice test can evaluate murine recognition and preference responses to different characteristics of USVs.

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Developmental Biology

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
Yu-Han Yeh *1,2, Mengwen Hu *1,2, Toshinori Nakagawa 3,4, Akihiko Sakashita 1,2, Shosei Yoshida 3,4, So Maezawa 5,6, Satoshi H. Namekawa 1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science

Here we present a simple and efficient method to isolate live meiotic and post-meiotic germ cells from adult mouse testes. Using a low-cytotoxicity, violet-excited DNA binding dye and fluorescence-activated cell sorting, one can isolate highly enriched spermatogenic cell populations for many downstream applications.

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Behavior

Analysis of Electrocardiograms and Behavior in Mice from Pregnancy to Lactation Period
Kie Shimizu 1, Sayaka Kuze-Arata 1, Takefumi Kikusui 1, Kazutaka Mogi 1
1Department of Animal Science and Biotechnology, Azabu University

A simultaneous recording of autonomic activity and detailed maternal behavior of mother mice from pregnancy to lactation was achieved using a telemetry system. This method helps to understand the dynamics of the physiological and behavioral characteristics in mothers from pregnancy to weaning.

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