JoVE Logo

Sign In

Blood Research Institute

5 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Depletion of Specific Cell Populations by Complement Depletion
Bonnie N. Dittel 1
1BloodCenter of Wisconsin, Blood Research Institute

To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.

image

Immunology and Infection

Isolation of Mouse Peritoneal Cavity Cells
Avijit Ray 1, Bonnie N. Dittel 1
1BloodCenter of Wisconsin, Blood Research Institute

The peritoneal cavity in mammals contains different immune cell populations crucial for innate immune responses. An efficient isolation method is required for biochemical and functional analyses of these cells. Here we provide a comprehensive method for the isolation of peritoneal cavity cells in the mouse.

image

Immunology and Infection

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
Sreemanti Basu 1, Hope M. Campbell 1, Bonnie N. Dittel 1, Avijit Ray 1
1Blood Research Institute, BloodCenter of Wisconsin

For many scientific studies requiring a biological and chemical analysis of cell populations the cells must be in a high state of purity. Fluorescence activated cell sorting (FACS) is a superior method in which to obtain pure cell populations.

image

Immunology and Infection

High-throughput Detection Method for Influenza Virus
Pawan Kumar 1, Allison E. Bartoszek 1, Thomas M. Moran 2, Jack Gorski 3, Sanjib Bhattacharyya 4, Jose F. Navidad 4, Monica S. Thakar 1,5, Subramaniam Malarkannan 1,6
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, 2Department of Microbiology, Mount Sinai School of Medicine , 3Laboratory of Molecular Genetics, Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children's Hospital of Wisconsin, Medical College of Wisconsin , 6Division of Hematology and Oncology, Dept Medicine, Medical College of Wisconsin

This method describes the use of Infrared dye based imaging system for detection of H1N1 in bronchioalveolar lavage (BAL) fluid of infected mice at a high sensitivity. This methodology can be performed in a 96- or 384-well plate, requires <10 μl volume of test material and has the potential for concurrent screening of multiple pathogens.

image

Immunology and Infection

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
Arash Nanbakhsh 1, Brad Best 2, Matthew Riese 3, Sridhar Rao 4, Li Wang 5, Jeffrey Medin 6, Monica S. Thakar 6, Subramaniam Malarkannan 1,5,6,7
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, 5Department of Microbiology and Immunology, The Medical College of Wisconsin, 6Department of Pediatrics, The Medical College of Wisconsin, 7Department of Medicine, The Medical College of Wisconsin

The goal of this study was to formulate technologies that allow for successful gene transduction in primary natural killer (NK) cells. The dextran-mediated lentiviral transduction of human or mouse primary NK cells results in higher gene expression efficiencies. This method of gene transduction will vastly improve NK cell genetic manipulation.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved