Core Research for Evolutionary Science and Technology (CREST)

5 ARTICLES PUBLISHED IN JoVE

Biology

¹Genetic Engineering and Functional Genomics Unit, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University

The light/dark transition test is one of the most widely used tests to measure anxiety-like behavior in mice. Here, we present a movie that shows detailed procedures on how we conduct the test.

Biology

Elevated Plus Maze for Mice

Munekazu Komada¹, Keizo Takao^1,2, Tsuyoshi Miyakawa²

¹Genetic Engineering and Functional Genomics Unit, Frontier Technology Center, Graduate School of Medicine, Kyoto University, ²Institute for Comprehensive Medical Science Division of Systems Medicine, Fujita Health University

The elevated plus maze test is one of the most widely used tests for measuring anxiety-like behavior in mice. Here, we present a movie showing the detailed procedures for conducting the test.

Biology

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Hideo Hagihara^1,2, Keiko Toyama^1,2, Nobuyuki Yamasaki^1,3, Tsuyoshi Miyakawa^1,2,4,5

¹Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST), ²Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, ³Department of Psychiatry, Graduate School of Medicine, Kyoto University, ⁴Genetic Engineering and Functional Genomics Group, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, ⁵Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

A dissection technique for removal of the dentate gyrus from adult mouse under a stereomicroscope was demonstrated in this video-recorded protocol.

Neuroscience

T-maze Forced Alternation and Left-right Discrimination Tasks for Assessing Working and Reference Memory in Mice

Hirotaka Shoji^1,2, Hideo Hagihara¹, Keizo Takao³, Satoko Hattori^1,2,3, Tsuyoshi Miyakawa^1,2,3

¹Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, ²Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST), ³Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

This article presents the protocol of T-maze tests using a modified automated apparatus for assessing the learning and memory functions in mice.

Behavior

Contextual and Cued Fear Conditioning Test Using a Video Analyzing System in Mice

Hirotaka Shoji^1,2, Keizo Takao^2,3, Satoko Hattori^1,2, Tsuyoshi Miyakawa^1,2,3

This article presents a protocol for a contextual and cued fear conditioning test using a video analyzing system to assess fear learning and memory in mice.