Federal Institute for Drugs and Medical Devices (BfArM)

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Non-restraining EEG Radiotelemetry: Epidural and Deep Intracerebral Stereotaxic EEG Electrode Placement

Anna Papazoglou¹, Andreas Lundt¹, Carola Wormuth¹, Dan Ehninger², Christina Henseler¹, Julien Soós¹, Karl Broich¹, Marco Weiergräber¹

¹Department of Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices (Bundesinstitut für Arzneimittel und Medizinprodukte, BfArM), ²Molecular and Cellular Cognition Lab, German Center for Neurodegenerative Diseases (Deutsches Zentrum für Neurodegenerative Erkrankungen, DZNE)

Non-restraining EEG radiotelemetry is a valuable methodological approach to record in vivo long-term electroencephalograms from freely moving rodents. This detailed protocol describes stereotaxic epidural and deep intracerebral electrode placement in different brain regions in order to obtain reliable recordings of CNS rhythmicity and CNS related behavioral stages.

Behavior

Automatic Detection of Highly Organized Theta Oscillations in the Murine EEG

Ralf Müller¹, Anna Papazoglou², Julien Soos², Andreas Lundt², Carola Wormuth², Christina Henseler², Dan Ehninger³, Karl Broich⁴, Marco Weiergräber²

¹Department of Psychiatry and Psychotherapy, University of Cologne, ²Department of Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices, ³Molecular and Cellular Cognition Lab, German Center for Neurodegenerative Diseases, ⁴Federal Institute for Drugs and Medical Devices

Theta activity in the hippocampus is related to specific cognitive and behavioral stages. Here, we describe an analytical method to detect highly-organized theta oscillations within the hippocampus using a time-frequency (i.e., wavelet analysis)-based approach.

Neuroscience

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

Andreas Lundt^1,2, Julien Soos¹, Christina Henseler¹, Muhammad Imran Arshaad¹, Ralf Müller³, Dan Ehninger⁴, Jürgen Hescheler⁵, Agapios Sachinidis⁵, Karl Broich⁶, Carola Wormuth^1,7, Anna Papazoglou¹, Marco Weiergräber¹

¹Experimental Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices, ²KBRwyle GmbH, ³Cognitive Neurophysiology, Department of Psychiatry and Psychotherapy, Faculty of Medicine, University of Cologne, ⁴Molecular and Cellular Cognition, German Center for Neurodegenerative Diseases (DZNE), ⁵Institute of Neurophysiology, Faculty of Medicine, University of Cologne, ⁶Federal Institute for Drugs and Medical Devices (BfArM), ⁷Thescon GmbH

Brainstem evoked response audiometry is an important tool in clinical neurophysiology. Nowadays, brainstem evoked response audiometry is also applied in the basic science and preclinical studies involving both pharmacological and genetic animal models. Here we provide a detailed description of how auditory brainstem responses can be successfully recorded and analyzed in mice.