Institute of Complex Molecular Systems (ICMS)

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Sandra Van de Wiel¹, Maarten Merkx^2,3, Stan Van de Graaf¹

¹Tytgat Institute for Liver and Intestinal Research, Department of Gastroenterology and Hepatology, Academic Medical Center, ²Laboratory of Chemical Biology, Institute of Complex Molecular Systems (ICMS), ³Department of Biomedical Engineering, Eindhoven University of Technology

We provide a detailed protocol to study bile acid dynamics in living cells using a genetically encoded BAS FRET sensor. This Bile Acid Sensor represents a unique tool to study (regulation of) bile acid transport and FXR activation in a wide range of cell types.