LYSSA LLC
2 ARTICLES PUBLISHED IN JoVE
JoVE Methods Collections: Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control
Charles E. Rupprecht 1, Wei-Cheng Hsu 2, Feng Ye 3, Megan Golding 4, Luca Zaeck 5, Laurent Dacheux 6, Noel Tordo 7, Jodie A. Jarvis 8, Erin Patrick 9, Are Berentsen 10
1LYSSA LLC, 2Animal Health Research Institute, Council of Agriculture, Executive Yuan, 3Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, 4Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, 5Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 6Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, 7Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus, Institut Pasteur, Institut Pasteur de Guinea, Conakry, 8Rabies Laboratory, Wadsworth Center, New York State Department of Health, 9Animal and Plant Health Inspection Service, Wildlife Services, Knoxville, TN, US Department of Agriculture, 10Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, US Department of Agriculture
JoVE Methods Collections: Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control
Immunology and Infection
Enhanced Rabies Surveillance Using a Direct Rapid Immunohistochemical Test
Erin M. Patrick 1, Brian M. Bjorklund 2, Jordona D. Kirby 3, Kathleen M. Nelson 4, Richard B. Chipman 4, Charles E. Rupprecht 5
1Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Knoxville, TN, United States Department of Agriculture (USDA), 2Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Sutton, MA, United States Department of Agriculture (USDA), 3Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Milton, FL, United States Department of Agriculture (USDA), 4Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Concord, NH, United States Department of Agriculture (USDA), 5Lyssa LLC
The direct rapid immunohistochemical test (DRIT) offers a World Organization for Animal Health and World Health Organization (OIE/WHO) recognized alternative to the direct fluorescent antibody (DFA) test for rabies diagnosis. This test allows for field-based applications that can be performed in approximately 1 h upon brain impressions using light microscopy.
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