Merck & Co., Inc

1 ARTICLES PUBLISHED IN JoVE

Medicine

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Shuangping Shi¹, Russ G.G. Condon¹, Liang Deng¹, Jason Saunders¹, Finn Hung¹, Yung-Shyeng Tsao¹, Zhong Liu¹

¹Merck Research Laboratory, Merck & Co., Inc

A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line quality and high reproducibility.