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Nexcelom Bioscience LLC

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
Charlotte Berkes 1,2, Leo Li-Ying Chan 2,3, Alisha Wilkinson 1,2,3, Benjamin Paradis 2,3
1Department of Biology, Merrimack College, 2Center for Biotechnology and Biomedical Sciences, Merrimack College, 3Department of Technology R&D, Nexcelom Bioscience LLC

Here, we demonstrate how image cytometry can be used for quantification of pathogenic fungi in association with host cells in culture. This technique can be used as an alternative to CFU enumeration.

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Cancer Research

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
Yongyang Huang 1, Jinyun Zou 1, Mudabbir Badar 1, Junchao Liu 1, Wentao Shi 5, Shunqiang Wang 2, Qiongyu Guo 3, Xiaofang Wang 1, Sarah Kessel 4, Leo Li-Ying Chan 4, Peter Li 4, Yaling Liu 2,5, Jean Qiu 4, Chao Zhou 1,5,6
1Department of Electrical and Computer Engineering, Lehigh University, 2Department of Mechanical Engineering, Lehigh University, 3Department of Biomedical Engineering, Southern University of Science and Technology, 4Department of Technology R&D, Nexcelom Bioscience LLC, 5Department of Bioengineering, Lehigh University, 6Center for Photonics and Nanoelectronics, Lehigh University

Optical coherence tomography (OCT), a three-dimensional imaging technology, was used to monitor and characterize the growth kinetics of multicellular tumor spheroids. Precise volumetric quantification of tumor spheroids using a voxel counting approach, and label-free dead tissue detection in the spheroids based on intrinsic optical attenuation contrast, were demonstrated.

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