Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in ZebrafishHua Wen 1, Paul Brehm 1
1Vollum Institute, Oregon Health and Sciences University
Larval zebrafish represent the first vertebrate model system to allow simultaneous patch clamp recording from a spinal motor-neuron and target skeletal muscle. This video demonstrates the microscopic methods used to identify a segmental CaP motor-neuron and target muscle cells as well as the methodologies for recording from each cell type.