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Shandong Academy of Agricultural Sciences

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
Xiao Xu 1, Yinwei You 1,2, Long Zhang 1
1Department of Entomology, China Agricultural University, 2Bio-tech Research Center, Shandong Academy of Agricultural Sciences

This paper describes a detailed and highly effective RNA in situ hybridization protocol particularly for low-level expressed Odorant Receptor (OR) genes, as well as other genes, in insect antennae using digoxigenin (DIG)-labeled or biotin-labeled probes.

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Neuroscience

Single Sensillum Recordings for Locust Palp Sensilla Basiconica
Hongwei Li 1, Yinwei You 2, Long Zhang 1
1Department of Entomology, China Agricultural University, 2Bio-tech Research Center, Shandong Academy of Agricultural Sciences

This paper describes a detailed and highly effective protocol for single sensillum recordings from the sensilla basiconica on the palps of insect mouthparts.

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Biochemistry

Evaluating the Effect of Pesticides on the Larvae of the Solitary Bees
Yingying Song 1, Ruiping Li 1,2, Lili Li 1, Fang Ouyang 3, Xingyuan Men 1
1Institute of Plant Protection, Shandong Academy of Agricultural Sciences, 2College of Plant Protection, Shandong Agricultural University, 3State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences

The present protocol explains a method to feed pesticide-contaminated provisions to the larvae of the solitary bees, Osmia excavata. The procedure examines the ecotoxicity of the pesticide to the larvae of the solitary bees.

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Behavior

Dissection and Grading of Ovarian Development in Wild-Type Female Insects
Lara Sindhu 1, Sen Guo 1, Yingying Song 1, Lili Li 1, Hongyign Cui 1, Wenxiu Guo 1, Suhong Lv 1, Yi Yu 1, Xingyuan Men 1
1Institute of Plant Protection, Shandong Academy of Agricultural Sciences

The protocol demonstrates a simple and easy dissection method, suitable for wild-type migratory female insects captured with searchlight traps. This technique can significantly clarify the same species by comparing both reproductive tissues, namely the mating sac and ovarian development of wild-type female insects.

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