Tennessee Valley Healthcare System

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

piggyBac Transposon System Modification of Primary Human T Cells

Sunandan Saha^1,2, Yozo Nakazawa³, Leslie E. Huye^4,5, Joseph E. Doherty^2,6, Daniel L. Galvan², Cliona M. Rooney^4,5,7, Matthew H. Wilson^1,2,4,8

¹Program in Translational Biology and Molecular Medicine, Baylor College of Medicine , ²Department of Medicine, Division of Nephrology, Baylor College of Medicine , ³Department of Immunology and Pathology, Shinshu University School of Medicine, ⁴Center for Cell and Gene Therapy, Baylor College of Medicine , ⁵Department of Pediatrics, Baylor College of Medicine , ⁶Program in Cell and Molecular Biology, Baylor College of Medicine , ⁷Department of Molecular Virology and Microbiology, Baylor College of Medicine , ⁸Michael E. DeBakey VA Medical Center

We describe a method to genetically modify primary human T cells with a transgene using the non-viral piggyBac transposon system. T cells modified to using the piggyBac transposon system exhibit stable transgene expression.

Biology

Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney

Lauren E. Woodard^1,2,3, Richard C. Welch², Felisha M. Williams², Wentian Luo², Jizhong Cheng³, Matthew H. Wilson^1,2,3

¹Department of Veterans Affairs, Tennessee Valley Healthcare System, ²Departments of Medicine and Pharmacology, Vanderbilt University Medical Center, ³Department of Medicine, Baylor University College of Medicine

This protocol describes a method to inject plasmid DNA into the mouse kidney via the renal pelvis to produce transgene expression specifically in the kidney.