The Chinese University of Hong Kong, Prince of Wales Hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

Monitoring of Systemic and Hepatic Hemodynamic Parameters in Mice

Chichi Xie^1,3, Weiwei Wei¹, Tao Zhang¹, Olaf Dirsch², Uta Dahmen¹

¹Department of General, Visceral and Vascular Surgery, Jena University Hospital, ²Institute of Pathology, Jena University Hospital, ³Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University

This film demonstrates how to acquire systemic and hepatic hemodynamics in mice. The whole monitoring includes acquisition of vital parameters, systemic blood pressure, central venous pressure, common hepatic artery flow rate, and portal vein pressure as well as the portal flow rate in mice.

JoVE Core

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage

Yiwei Zhao¹, Gene Chi Wai Man^1,2, Loucia Kit Ying Chan¹, Xi Guo¹, Yingyu Liu³, Tao Zhang¹, Joseph Kwong^1,4, Chi Chiu Wang^1,5, Xiaoyan Chen³, Tin Chiu Li¹

¹Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, ²Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, ³Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital, Shenzhen University, ⁴School of Medicine, Faculty of Medicine and Health Sciences, Keele University, ⁵Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences, The Chinese University of Hong Kong

Despite the advancements in multiplex immunohistochemistry and multispectral imaging, characterizing the density and clustering of major immune cells simultaneously in the endometrium remains a challenge. This paper describes a detailed multiplex staining protocol and imaging for the simultaneous localization of four immune cell types in the endometrium.