The First Affiliated Hospital of Chongqing Medical University

2 ARTICLES PUBLISHED IN JoVE

Medicine

Surgical Models of Gastroesophageal Reflux with Mice

Jinxi He^1,2, Yu Fang^2,3, Xiaoxin Chen^2,4

¹Department of Thoracic Surgery, Ningxia Medical University General Hospital, ²Cancer Research Program, North Carolina Central University, ³Department of Thoracic & Cardiovascular Surgery, The First Affiliated Hospital of Chongqing Medical University, ⁴Department of Medicine, Center for Esophageal Disease and Swallowing, Division of Gastroenterology and Hepatology, University of North Carolina at Chapel Hill

This article demonstrates surgical procedures of gastroesophageal reflux with mice. These models are useful tools for research on mechanisms and treatment of gastroesophageal reflux disease and potentially Barrett’s esophagus and esophageal adenocarcinoma.

Neuroscience

Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

Jianjun Zhong^1,2, Georgia Gunner³, Nils Henninger¹, Dorothy P. Schafer³, Daryl A. Bosco¹

¹Department of Neurology, University of Massachusetts Chan Medical School, ²Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, ³Department of Neurobiology, University of Massachusetts Chan Medical School

This study demonstrates delivery of a repetitive traumatic brain injury to mice and simultaneous implantation of a cranial window for subsequent intravital imaging of a neuron-expressed EGFP using two-photon microscopy.