Universidad de Buenos Aires

4 ARTICLES PUBLISHED IN JoVE

Behavior

Probing the Limits of Egg Recognition Using Egg Rejection Experiments Along Phenotypic Gradients

Lindsay Canniff¹, Miri Dainson², Analía V. López³, Mark E. Hauber², Tomáš Grim⁴, Peter Samaš⁵, Daniel Hanley¹

¹Department of Biology, Long Island University-Post, ²Department of Animal Biology, School of Integrative Biology, University of Illinois, ³Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, ⁴Department of Zoology and Laboratory of Ornithology, Palacký University, ⁵Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic

This protocol provides guidelines for running egg rejection experiments: outlining techniques for painting experimental egg models to emulate the colors of natural bird eggs, conducting fieldwork, and analyzing the collected data. This protocol provides a uniform method for conducting comparable egg rejection experiments.

Bioengineering

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Marcos Ezequiel Gramajo^*1, Ryan J. Lake^*2, Yi Lu³, Ana Sol Peinetti¹

¹INQUIMAE (CONICET), Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, ²Department of Chemistry, University of Illinois at Urbana-Champaign, ³Department of Chemistry, University of Texas at Austin

We provide a protocol that can be generally applied to select aptamers that bind to infectious viruses only and not to viruses that have been rendered non-infectious by a disinfection method or to any other similar viruses. This opens the possibility of determining infectivity status in portable and rapid tests.

Medicine

An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice

Florencia S. Fontana Estevez^*1, Verónica Miksztowicz^*1, Ignacio M. Seropián^1,2, Pablo Cassaglia³, Romina Bustos¹, Vanessa Touceda¹, Tomas Cianciulli⁴, Verónica Cassanova⁵, Celina Morales³, Germán E. González¹

¹Laboratorio de Patología Cardiovascular Experimental e Hipertensión Arterial, Instituto de Investigaciones Biomédicas, Universidad Católica Argentina-CONICET, ²Servicio de Hemodinamia y Cardiología Intervencionista, Hospital Italiano de Buenos Aires, ³Instituto de Fisiopatologia Cardiovascular, Facultad de Medicina, Universidad de Buenos Aires, ⁴División Cardiología, Hospital General de Agudos "Dr. Cosme Argerich,", ⁵Facultad de Veterinaria, Universidad de Buenos Aires

Here, we describe an experimental model of myocardial infarction, an echocardiography procedure to study cardiac remodeling and function, and procedures for quantifying fibrosis and hypertrophy in picrosirius red-stained and rhodamine-stained sections, as well as the infarct size and expansion index in slices stained with Masson's trichrome.

Developmental Biology

Early Unguided Human Brain Organoid Neurovascular Niche Modeling into the Permissive Chick Embryo Chorioallantoic Membrane

Luciano Fiore^1,2, Jan Arderiu³, Andrea Martí-Sarrias^3,4, Isabel Turpín^3,4, Ruth I. Pareja^3,5, Arcadi Navarro^5,6,7,8, Mariana Holubiec^2,9, Julieta Bianchelli⁹, Tomas Falzone^2,9, Gonzalo Spelzini^1,2, Gabriel Scicolone^1,2, Sandra Acosta^3,4

¹Instituto de Biología Celular y Neurociencias “Prof. E. De Robertis” (IBCN), CONICET - Universidad de Buenos Aires, ²Facultad de Medicina, Departamento de Biología Celular, Histología, Embriología y Genética, Universidad de Buenos Aires, ³Institute of Neurosciences, Pathology and Experimental Therapeutics Dept, University of Barcelona, ⁴Functional Neurogenomics Group, Neurodevelopmental Disorders, IDIBELL, L’Hospitalet de Llobregat, ⁵IBE, Institute of Evolutionary Biology (UPF-CSIC), Department of Medicine and Life Sciences, Universitat Pompeu Fabra, ⁶Institució Catalana de Recerca i Estudis Avançats (ICREA) and Universitat Pompeu Fabra, ⁷Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, ⁸BarcelonaBeta Brain Research Center, Pasqual Maragall Foundation, ⁹Instituto de Investigación en Biomedicina (IBioBA) – CONICET – Instituto Partner de la Sociedad Max Planck

Here, we present a protocol to engraft human brain organoids at multiple maturation stages into the chick chorioallantoic membrane (CAM). Brain organoids were grown following unguided standardized protocols.