University of California San Francisco and San Francisco General Hospital

5 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

Kevin Wilhelmsen¹, Katherine Farrar^1,2, Judith Hellman¹

¹Department of Anesthesia and Perioperative Care, University of California, San Francisco, ²Graduate Program in Biomedical Sciences, University of California, San Francisco

Neutrophil adherence to the activated endothelium at sites of infection is an integral component of the host's inflammatory response. Described in this report is a neutrophil binding assay that allows for the in vitro quantitation of primary human neutrophil binding to endothelial cells activated by inflammatory mediators under static conditions.

Biology

Oral Bacterial Infection and Shedding in Drosophila melanogaster

Jonathon A. Siva-Jothy¹, Arun Prakash¹, Radhakrishnan B. Vasanthakrishnan², Katy M. Monteith¹, Pedro F. Vale^1,3

¹Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, ²IGDR - CNRS UMR 6290, ³Centre for Immunity, Infection and Evolution, University of Edinburgh

This protocol describes methods to orally expose and infect the fruit fly Drosophila melanogaster with bacterial pathogens, and to measure the number of infectious bacteria shed following gut infection. We further describe the effect of immune mutants on fly survival following oral bacterial infection.

Immunology and Infection

Isolation of Primary Mouse Lung Endothelial Cells

Erika Wong^1,2, Nina Nguyen², Judith Hellman²

¹Department of Pediatrics, Division of Critical Care, UCSF Benioff Children's Hospital, ²Department of Anesthesia and Perioperative Care, University of California, San Francisco

In this article, primary lung endothelial cells were isolated and cultured from neonatal mice.

Immunology and Infection

A Mouse Model of Orotracheal Intubation and Ventilated Lung Ischemia Reperfusion Surgery

Wen-I Liao^*1, Daisuke Maruyama^*1, Farzaneh Kianian^1,2, Christine Tat¹, Xiaoli Tian¹, Judith Hellman¹, Jeffrey M Dodd-o³, Arun Prakash¹

¹Department of Anesthesia and Perioperative Care, University of California San Francisco and San Francisco General Hospital, ²Department of Physiology, School of Medicine, Tehran University of Medical Sciences, ³Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine

A mouse surgical model to create left lung ischemia reperfusion (IR) injury while maintaining ventilation and avoiding hypoxia.

Immunology and Infection

Sequential Blood Collection from Inferior Vena Cava Followed by Portal Vein to Evaluate Gut Microbial Metabolites in Mice

Thien N. M. Doan¹, Daisuke Maruyama¹, Xiaoli Tian¹, Arun Prakash¹

¹Department of Anesthesia and Perioperative Care, University of California San Francisco and San Francisco General Hospital

The protocol demonstrates a method to collect blood from portal veins and inferior vena cava from mice sequentially to evaluate the production and absorption of gut microbial metabolites.