University of Colorado Anschutz

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Manufacturing and Using Piggy-back Multibarrel Electrodes for In vivo Pharmacological Manipulations of Neural Responses

Anna Dondzillo¹, Jennifer L. Thornton¹, Daniel J. Tollin¹, Achim Klug¹

¹Department of Physiology & Biophysics, University of Colorado Medical Campus

Iontophoresis of neural agonists and antagonists during extracellular in vivo recordings is a powerful way to manipulate a neuron’s microenvironment. These manipulations can most easily be done via piggy-back multibarrel electrodes. Here we describe how to manufacture them and use them during auditory recordings.

Neuroscience

Laser-guided Neuronal Tracing In Brain Explants

Otto Albrecht¹, Achim Klug¹

¹Department of Physiology and Biophysics, University of Colorado School of Medicine

We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.

Neuroscience

Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices

Elizabeth A. McCullagh¹, Shani Poleg², Dominik Stich³, Radu Moldovan^*3, Achim Klug^*2

¹Department of Integrative Biology, Oklahoma State University, ²Department of Physiology and Biophysics, University of Colorado Anschutz, ³Advanced Light Microscopy Core, University of Colorado Anschutz

Visualizing myelination is an important goal for many researchers studying the nervous system. CARS is a technique that is compatible with immunofluorescence that can natively image lipids within tissue such as the brain illuminating specialized structures such as myelin.