High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human SerumMazen Amatoury 1, Vera Merheb 1, Jessica Langer 1, Xin Maggie Wang 2, Russell Clive Dale 1, Fabienne Brilot 1
1Institute for Neuroscience and Muscle Research, The Kids Research Institute at the Children's Hospital at Westmead, The University of Sydney, 2Flow Cytometry Centre, Westmead Millennium Institute for Medical Research
Over the recent years, live cell-based assays have been used successfully to detect antibodies against surface and conformational antigens. Here, we describe a method using high-throughput flow cytometry enabling the analysis of large cohorts of patients. Detection of novel antibodies will improve diagnosis and treatment of immune-mediated disorders.
