This method can help to answer the key questions in the microfluidic droplet formation field, including fabricating the semi-three dimensional flow focusing PDMS chip, and producing the pure PGD droplets. The main advantage of this tactic is that
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Here, we present a protocol to illustrate the fabrication processes and verifying experiments of a semi-three-dimensional (semi-3D) flow-focusing microfluidic chip for droplet formation.