Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

0:25

Inducing PCD (protocatechuate 3,4 dioxygenase) Expression in E. coli

3:39

Nickel Affinity Chromatography Purification of PCD

4:46

Nuclease Activity Assay

6:19

PCA (Protocatechuic Acid) Oxidation and Nuclease Activity Assays

8:01

Results: Purification of PCD

9:23

Conclusion