Cyclophosphamide Myeloablation and Human Peripheral Blood Mononuclear Cell (PBMC) Transplantation and Tumor Engraftment
2:08
Immunohistochemistry (IHC)
4:33
In Vivo Efficacy and Pharmacodynamics Analyses
6:08
Results: Representative PBMC- and Tumor-Engraftment and Anti-Tumor Antibody Treatment Analysis
7:16
Conclusion
Transkript
Our protocol can serve as a general guideline for establishing mouse models reconstituted with human PBMC and tumors for immuno-oncology research. These procedures provide a cost-effective and highly reproducible approach for partially reconstitut
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We describe a human peripheral blood mononuclear cell (PBMC) — based humanized xenograft mouse model for translational immuno-oncology research. This protocol could serve as a general guideline for establishing and characterizing similar models for I-O therapy assessment.